Evaluation of a commercial latex agglutination assay for serological diagnosis of leptospirosis.

Diagnosis of Leptospirosis Agglutination Assay for Serological Evaluation of a Commercial Latex

Comparison of Two ELISA Methods for the Laboratory Diagnosis of Acute Leptospirosis

Background: Leptospirosis is the most common zoonosis widespread in tropical and temperate countries with low social-economic status. We aimed to compare an ELISA kit with an in-house ELISA assay to test the serum samples of the patients who were suspicious of leptospirosis according to their clinical symptoms. Methods: A total of 282 serum samples of patients suspicious of leptospirosis admitt...

Preparation and evaluation of latex agglutination technique for diagnosis and seroepidemiological studies of visceral leishmaniasis

in this study which has been carried out from 1996 until 1997 soluble latex agglutination antigen was prepared from L, donovani,sudan(s-1) by culturing in special media,harvesting,and later solubilizing by freezing and thawing.the antigen were futher prepared by high speed centrifugation.after measurement of the protein (lowry method) concentration of the antigen was set at 2.2 mg/ml,the antige...

Development of a Latex Agglutination Method for Diagnosis of Rotavirus Infections

Background: Rotavirus is a major cause of morbidity and mortality among children with gastroenteritis. Since the discovery of rotaviruses, several techniques have been used for their laboratory diagnosis; those included Electron Microscopy (EM) and enzyme immunoassay. These methods, however, are expensive and not readily available everywhere. We have developed a technique which can be used for ...

Evaluation of recombinant leptospiral antigen LipL41 in enzyme- linked immunosorbent assay and latex agglutination test for serodiagnosis of canine leptospirosis

Leptospirosis is an important global veterinary health problem caused by spirochetes belonging to the genus Leptospira. Leptospires are ubiquitous in nature, and therefore able to adapt to both the ambient environment and the renal tubules of chronically infected reservoir hosts. Microscopic agglutination test (MAT) remains a specialized test, which is not generally performed in the routine dia...